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货号： 4371353

描述:

The Applied Biosystems® TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

• Distinct clusters and high call rates for unambiguous allelic discrimination 

• Validated with TaqMan® SNP Genotyping Assays 

• Excellent pre- and post-PCR stability for high throughput setup and analysis

May be used for a variety of genotyping applications, including:

• Candidate gene studies

• Drug-target validation 

• Disease association studies

• Population genetics

• Linkage mapping

• Agricultural applications

Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)

Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup. 

• Optimized mix components provide excellent specificity for discrimination between alleles 

• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments 

• Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays and Instruments

TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.

High-Throughput Setup and Analysis

The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend. 

Reliable Discrimination with Challenging Targets

Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality

Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').