QQ交谈
您所在位置：请输入产品关键字：
邮编：201203
联系人：杨经理
电话：021-50724187 021-50724961
传真：021-50724961
手机：15921799099
留言：发送留言
个性化：www.qfbio.com
网址：www.qfbio.com
商铺：https://www.chem17.com/st178406/
pPIC3.5K载体说明书
最近更新时间：2013-8-9
提 供 商：上海起发实验试剂有限公司资料大小：465.3KB
文件类型：PDF 文件下载次数：452次
资料类型：浏览次数：3848次
pPIC3.5K
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VJI0298 | pPIC3.5K | Invitrogen | 酵母表达载体 |
Multiple copy integration of recombinant genes in Pichia has been demonstrated to
increase expression of the desired gene in some cases （Brierley, et al., 1994; Clare, et al.,
1991a; Cregg, et al., 1993; Romanos, et al., 1991; Scorer, et al., 1993; Scorer, et al.,
1994; Thill, et al., 1990; Vedvick, et al., 1991）. The two vectors included in this kit
allow isolation and generation of multicopy inserts, either by in vivo or in vitro methods,
in order to test whether increasing the copy number of your recombinant gene will lead to
a subsequent increase in protein expression. The in vivo method utilizes hyper-resistance
to GeneticinÆ
（G418 sulfate） to screen for possible multicopy inserts, while the in vitro
method produces tandem inserts of your gene by ligation.
