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pPIC3.5K载体说明书
最近更新时间：2013-8-9
提 供 商：上海起发实验试剂有限公司资料大小：465.3KB
文件类型：PDF 文件下载次数：452次
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pPIC3.5K
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VJI0298 | pPIC3.5K | Invitrogen | 酵母表达载体 |
Multiple copy integration of recombinant genes in Pichia has been demonstrated to
increase expression of the desired gene in some cases （Brierley, et al., 1994; Clare, et al.,
1991a; Cregg, et al., 1993; Romanos, et al., 1991; Scorer, et al., 1993; Scorer, et al.,
1994; Thill, et al., 1990; Vedvick, et al., 1991）. The two vectors included in this kit
allow isolation and generation of multicopy inserts, either by in vivo or in vitro methods,
in order to test whether increasing the copy number of your recombinant gene will lead to
a subsequent increase in protein expression. The in vivo method utilizes hyper-resistance
to GeneticinÆ
（G418 sulfate） to screen for possible multicopy inserts, while the in vitro
method produces tandem inserts of your gene by ligation.
