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毕赤酵母表达系统说明书
最近更新时间:2013-8-9
提 供 商:上海起发实验试剂有限公司资料大小:477KB
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毕赤酵母表达系统说明书
You can put the power of P. pastoris into your laboratory with a complete Pichia Expression Kit or with individual Pichia expression vectors. Three Pichia Expression Kits are available, each of which includes vectors, P. pastoris strains, reagents for transformation, sequencing primers, media, and a comprehensive manual (Table 3). In addition, the pPICZ, pGAPZ, and pPIC6 vectors are available separay (see insert for ordering information). Choose the kit or vector that best meets your research needs and take advantage of the proven strength of P. pastoris.
Table 3—Pichia expression kit components.
EasySelect™ Pichia
Expression Kit
Multi-Copy Pichia
Expression Kit
Original Pichia
Expression Kit
Vectors
pPICZ A, B, C
(20 µg each)
pPICZα A, B, C
(20 µg each)
pPIC9K (20 µg)
pPIC3.5K (20 µg)
pAO815 (20 µg)
pPIC9 (10 µg)
pPIC3.5 (10 µg)
pHIL-D2 (10 µg)
pHIL-S1 (10 µg)
Strains
X-33 (Mut+
, His+
)
GS115 (Mut+
)
KM71 (MutS
)
GS115/pPICZ/lacZ
(control)
GS115 (Mut+
)
KM71 (MutS
)
GS115/β-gal (control)
GS115/albumin (control)
GS115 (Mut+
)
KM71 (MutS
)
GS115/β-gal (control)
GS115/albumin (control)
Transformation
reagents
Pichia EasyComp™ Kit
(120 transformations)
Spheroplast Module
(50 transformations)
Spheroplast Module
(50 transformations)
Sequencing
primers
5´ AOX1
3´ AOX1
α-factor
5´ AOX1
3´ AOX1
α-factor
5´ AOX1
3´ AOX1
α-factor
Media and
supplements
YP base medium
(2 pouches)*
YP base agar
(2 pouches)*
Yeast Nitrogen Base
(1 pouch)†
Zeocin™ (250 mg)
YP base medium
(2 pouches)*
YP base agar
(2 pouches)*
Yeast Nitrogen Base
(1 pouch)†
YP base medium
(2 pouches)*
YP base agar
(2 pouches)*
Yeast Nitrogen Base
(1 pouch)†
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