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pYD1下载说明书
最近更新时间：2013-8-9
提 供 商：上海起发实验试剂有限公司资料大小：288.5KB
文件类型：PDF 文件下载次数：478次
资料类型：浏览次数：3555次
pYD1
Host：Yeast
Tags：V5 epitope; polyhistidine （6xHis） tag
pYD1为酒精酵母菌表面展示载体，可以通过锚定蛋白将目的蛋白大量展示到酵母细胞壁中，操作简便，产量高。
The pYD1 Yeast Display Vector is specifically designed to target and display recombinant proteins on the surface of Saccharomyces cerevisiae. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. pYD1 uses the a-agglutinin yeast adhesion receptor which consists of two domains, Aga1 and Aga2, to display recombinant protein on the surface of S. cerevisiae. The gene of interest is cloned into the pYD1 vector in frame with the AGA2 gene. The resulting construct is transformed into the EBY100 S. cerevisiae strain （supplied transformed with AGA1）. Aga1 and the Aga2-fusion protein associate within the secretory pathway and are displayed on the cell surface.
