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pUB6/V5 His B说明书
最近更新时间：2013-8-12
提 供 商：上海起发实验试剂有限公司资料大小：304.8KB
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pUB6/V5 His B
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VPI0023 | pUB6/V5 His B | Invitrogen | 哺乳动物表达载体 |
Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His）
Constitutive or Inducible System：Constitutive
pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.
The vectors contain the following elements:
- Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
- Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
- Blasticidin resistance gene （bsd） for selection of stable cell lines.
- Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.
The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
