iSTEM小鼠胚胎干细胞培养基是一款的小鼠胚胎干细胞维持培养基，还可以从难获得ES细胞的小鼠品系中获取小鼠ES细胞系。培养基添加3种小分子抑制剂阻断分化，扩增性能相比添加了LIF和BMP的培养基更*。
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英文名称：Cellartis iSTEM Embryonic Stem Cell Culture medium
中文名称：Cellartis iSTEM小鼠胚胎干细胞培养基
用途：小鼠胚胎干细胞维持培养
特别应用：从难获得ES细胞的小鼠品系中获取小鼠ES细胞系
保存：-20ºC
iSTEM是一款的小鼠胚胎干细胞维持培养基。该款产品包含三种小分子抑制物，从而阻断干细胞分化的信号通路，同时维持细胞的多能干性（pluripotency）。 iSTEM可以被应用于难以获得ES细胞的小鼠品系，获取到ES细胞系(1)。
iSTEM培养基中的小鼠ES细胞与在添加了Leukemia Inhibitory Factor (LIF) 和 serum/Bone Morphogenetic Protein (BMP)的培养基的细胞相比，增殖速度加倍(1)。另外，在iSTEM中培养的小鼠ES细胞产生细胞聚落（clone）的能力显著提高（enhanced clonogenicity）,从而可以在单细胞铺板后（single cell deposition），产生更多的干细胞克隆。
iSTEM is a compley defined cell culture medium for deriving, maintaining, and propagating mouse embryonic stem (ES) cells. This media formulation captures mouse ES cells in their basal state by blocking the inductive pathways of differentiation with selective small molecule inhibitors. The medium contains three small molecule inhibitors that block differentiation-inducing signals and promote cell survival, enabling maintenance of pluripotency without the need for additional stimulatory cytokines. iSTEM medium can also be used to derive mouse ES lines from strains that have proven refractory to ES cell derivation under standard conditions (1).
Mouse ES cells cultured in iSTEM medium continually expand at a doubling rate that is comparable to that of cells cultured in the presence of Leukemia Inhibitory Factor (LIF) and serum/Bone Morphogenetic Protein (BMP) (1). In addition, culture in iSTEM medium enhances clonogenicity; a higher frequency of pluripotent colonies are established after single cell deposition.
产品特点
*确定的配方：无血清，无饲养层和无细胞因子
使用选择性小分子抑制剂来阻断分化信号并将ES细胞维持在多能状态
使用iSTEM培养基，小鼠ES细胞可以有效地维持并以≥99％的纯度扩增
Compley defined formulation: serum-free, feeder-free, and cytokine-free
Uses selective small-molecule inhibitors to block differentiation signals and maintain ES cells in a pluripotent state
Using iSTEM medium, mouse ES cells can be efficiently maintained and propagated at ≥99% purity
产品应用
保持小鼠ES细胞的多能状态
从难获得ES细胞的(non-permissive)小鼠品系中获取小鼠ES细胞系
Maintaining mouse ES cells in a pluripotent state
Deriving mouse ES cell lines from non-permissive mouse strains
组份：培养基+添加剂
100 ml medium
100 µl supplement
自备试剂：
For mouse ES cells
Use culture vessels precoated with 0.1% gelatin type A in PBS (at room temperature for 15 minutes or more), or precoated sequentially with 0.01%poly-L-ornithine hydrobromide (at 37℃ for 30 minutes or more), washing twice with PBS, followed by 10 μg/mL laminin in PBS (at 37℃ for 3 hours or more).
对于小鼠ES细胞
使用在PBS中预涂有0.1％明胶A型的培养容器（在室温下15分钟或更长时间），或者依次用0.01％聚-L-鸟氨酸氢溴酸盐（在37℃下30分钟或更多）预涂覆，用PBS洗涤两次 ，然后用PBS中的10μg/ mL层粘连蛋白（37℃下3小时以上）。
保存：Upon receipt, store at -20℃ until ready to use. When stored under these conditions,the product is stable for 12 months from the date of manufacture (see label).Once thawed, store at 4℃ and use within 2 weeks.This product is light sensitive, and should be protected from light.
参考文献：
Ying, Q. L. et al. (2008) The ground state of embryonic stem cell self-renewal. Nature 453:519–523.
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iSTEM™ 小鼠胚胎干细胞培养基，难获得ES细胞小鼠
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华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司主营细胞治疗和精准医疗等产品，2018年与TAKARA继续合作，授权代理（上海）其旗下Cellartis干细胞研究制品和Rubicon单细胞文库构建试剂。Cellartis公司位于瑞典，拥有iPS细胞等干细胞分化为肝细胞及脏器细胞的分化诱导技术和ES细胞，ips细胞，分化细胞等干细胞相关产品。
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